Automatic oligonucleotide design for PCR-based gene synthesis
The availability of sequences of entire genomes has dramatically increased the number of protein targets, many of which will need to be overexpressed in cells other than the original source of DNA. Gene synthesis often provides a fast and economically efficient approach. The synthetic gene can be optimized for expression and constructed for easy mutational manipulation without regard to the parent genome. Yet design and construction of synthetic genes, especially those coding for large proteins, can be a slow, difficult and confusing process. DNAWorks automates the design of oligonucleotides for gene synthesis by PCR-based methods.
A description of the original DNAWorks can be found in our publication (Hoover, D. and Lubkowski, J., 2002).
For further improvements in the performance of DNAWorks, feedback on the success or failure of gene synthesis using DNAWorks is very important. Please let us know about the outcome of your synthesis experiments.
DNAWorks is open-source, and can be downloaded from https://github.com/davidhoover/DNAWorks. If you can find a a Fortran compiler, you can run DNAWorks via commandline.
Choice 1 - Upload sequence file:
Choice 2 - Enter sequence manually: