National Heart, Lung, and Blood Institute

Epithelial Systems Biology Laboratory (ESBL)


virtualBlot is a Java-based standalone command line program to generate the "virtual immunoblots" from Mass Spectometry data obtained from sliced SDS-PAGE gels. VirtualBlot takes all peptide intensities from the quantified LC-MS/MS as input data. The program employs the jHeatChart java module to generate the blot images.

This program was developed by Pumipat Tongyoo and Mark A. Knepper.

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Download virtualBlot sample data

Download virtualBlot instruction