Brief Method: PKA plays a crucial role in vasopressin signaling of renal collecting duct cells. To understand regulation of mRNA expression mediated by vasopressin/PKA signaling, mRNA expression was profiled by RNA-Seq in double knockout cells (both PKA catalytic genes) generated from mouse cortical collecting duct mpkCCD cell line and compared with control lines with intact PKA expression.
Reference: Isobe K, Jung HJ, Yang CR, Claxton J, Sandoval P, Burg MB, Raghuram V, Knepper MA. Systems-level identification of PKA-dependent signaling in epithelial cells. Proc Natl Acad Sci U S A. 2017 Oct, PMID: 28973931
Links to Data:
1. Data table for RNA-Seq quantification.2. Santa Cruz Genome Browser Mirror - Display of mapped reads in the mm10 genome.