Brief Method: Vasopressin signaling regulates transcription of several genes including water channel protein aquaporin-2 in the renal collecting duct. However, transcriptional regulation in downstream of vasopressin signaling is complicated due to the cell-type-specific expression and role of transcription factors and coregulators. We carried out ChIP-Seq for two b-ZIP transcription factors CREB (Gene symbol: Creb1) and C/EBP beta (Gene symbol: Cebpb) to identify genomic binding sites of these transcription factors in mouse cortical collecting duct cell line mpkCCD. CREB and C/EBP beta are known to be expressed in the renal collecting duct and regulated by vasopressin signaling (nuclear translocation and phosphorylation).
Reference: Jung HJ, Raghuram V, Lee JW, Knepper MA. Genome-Wide Mapping of DNA Accessibility and Binding Sites for CREB and C/EBPβ in Vasopressin-Sensitive Collecting Duct Cells. J Am Soc Nephrol. 2018;29(5):1490-1500. PubMed PMID: 29572403
Links to Data:
1. Data table for genes with transcription factor-binding site
2. UCSC Genome Browser Mirror - Display of mapped reads in the mm10 genome.