AbDesigner is a tool for analyzing the amino acid sequence of a given protein to identify optimal immunizing peptides for production of antibodies.
AbDesigner displays the information needed for choice of immunizing peptides, allowing the user to recognize trade-offs between immunogenicity, specificity, animal species targets, and post-translational modifications.
AbDesigner was developed by Trairak Pisitkun, Jason D. Hoffert, Fahad Saeed, and Mark A. Knepper at the Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute in Bethesda, Maryland USA. For problems or suggestions contact us.
Frequently Asked Questions
Disclaimer: Recommended peptides are not guaranteed to successfully produce antibodies. Users should consider all factors when planning an antibody production project including the conjugation method, immunization protocol, and antibody purification procedure.
Accessibility: Because of the complex intersections and simultaneous display protein sequences, using the AbDesigner requires the use of a mouse, and therefore presents known accessibility issues. The NHLBI is committed to providing this information for people who cannot use the tool as designed. For more information or assistance please contact Dr. Trairak Pisitkun at 301-496-8319, firstname.lastname@example.org or Dr. Mark Knepper at 301-496-3064, email@example.com.
Reference: If you use AbDesigner in any published work, please cite: Pisitkun T, Hoffert JD, Saeed F, Knepper MA. NHLBI-AbDesigner: an online tool for design of peptide-directed antibodies. Am J Physiol Cell Physiol. 2012 Jan;302(1):C154-64.
AbDesigner is hosted and supported by: